Then, the optical density (OD)260/280 values of the extracted RNA samples were measured with an ultraviolet spectrophotometer. operation group was increased compared to that in the control group; inhibition of miR-126 expression had a reversal effect, to a certain extent, on MVD increase after TACE (all em P /em 0.05). Inhibition of miR-126 expression increased Spred1 expression and decreased vascular endothelial growth factor expression ( em P /em 0.01). In summary, this study unveiled the potential mechanism by which miR-126 regulates angiogenesis in HCC tissues through embolization treatment by targeting Spred1, and also showed that the feasibility of TACE with the miR-126 inhibitor has a certain value in the medical treatment of HCC. strong class=”kwd-title” Keywords: microRNA-126, sprouty-related, EVH1 domain containing protein 1, hepatocellular carcinoma, transcatheter hepatic arterial HBEGF chemoembolization, animal modeling, angiogenesis, vascular endothelial growth factor, microvessel density Introduction Hepatocellular carcinoma (HCC) is currently regarded as one of the most malignant cancers with a high incidence.1 In the Peoples Republic of China, the annual HCC incidence is over 300,000, comprising 60% of the worlds HCC incidence.2 The current research suggested that the joint reactions between telomere shortening, chromosomal instability, and p21WAF1/CIP1 inactivation play important roles in HCC formation.3 There are some histologic changes in this process, including changes in blood supply, material changes of water, fat, iron, and other components, and changes of cell nodules in shape, size, and density.4 Surgical treatment is still an important means of HCC treatment. However, it has poor therapeutic effects and prognosis, mainly due to late diagnoses.1 HCC is a typical multivessel tumor, and antiangiogenic therapy has become a research focus Salubrinal and Salubrinal an important method for HCC treatment.5 Transcatheter arterial chemoembolization (TACE) therapy is a common nonsurgical antiangiogenesis treatment for HCC.6 MicroRNA (miRNA) is a noncoding, small-molecule RNA having a length of 20C24 nucleotides, which can match with its target genes inside a 3 noncoding region and inhibit the translation process of genes to accelerate its degradation.7 A recent study has found that miRNA can regulate the development of multiple decades of tumors through the rules of related genes expression levels of cell proliferation and apoptosis.8 Other studies have shown that there are some differentially indicated miRNAs in HCC tissues, which might be involved in a number of pathological processes within the generation, development, and metastasis of a tumor.9C11 These studies also show the miRNA abnormalities often exist before the occurrence of irregular gene regulation. Therefore, adequate studies of different specific miRNA-targeting genes and their regulatory mechanisms can contribute to the analysis or treatment of HCC.10,12 Evidence reported that sprouty-related, EVH1 website containing protein (Spred) family members, including Spred1 and Spred2, had low expressions in tumor cells, and were negatively correlated with tumor invasion and metastasis.13 MicroRNA-126 (miR-126), the angiogenesis-related miRNA, is regarded as one of the main regulators of physiological angiogenesis.14 Furthermore, miR-126 experienced effects on vascularization of placenta, and experienced significant angiogenic effects both Salubrinal in ischemia-induced angiogenesis in vivo and cultured endothelial cells in vitro.15,16 But some results of miRNA-related target site gene detection declared that miR-126 was one of the potential target genes of Spred1,17 which has not yet been confirmed by other relevant studies. Therefore, this study was focused on verification of the prospective relationship between miR-126 and Spred1, as well as their related effects on HCC angiogenesis after TACE. Materials and methods Honest statement This study was performed with the authorization of experimental Salubrinal animal ethic committee of Wenzhou Medical University or college. Further, we carried out this experiment based on the welfare and honest principles relating to Welfare and Ethics of Medical Laboratory Animals. All the methods involving subjects were performed in the study after obtaining the honest authorization of the Medical Ethics Committee of the University or college of Washington. All subjects provided written educated consent form. Study.
